Pub No:
122
Title:
Thin-layer Chromatography of Acid-labile Cobalamins
Authors:
Silverman, R.B. and David Dolphin
Journal:
J. Chromatogr.
Year:
1980
Pages:
194, 273-274. Vol. XX, 147-155
Abstract:
Acid-labile, axial ligand-substituted cobalamins were separated by thin-layer chromatography using (TLC) on cellulose under slightly basic conditions. The compounds were dissolved in 0.5% concentrated NH4OH and applied to 20 cm x 0.1 mm microcrystalline cellulose plates. Ascending thin-layer chromatography was performed at ambient temperature in the dark to a distance of approx.10 cm, and the solvent was BuOH-EtOH-H2O (10:3:7) containg 0.5% concentrated NH4OH. The compounds were visualized by their intense red color.

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