Pub No:
78
Title:
Application of HPLC to the Analysis of Clinically Important Porphyrins”, in "High Pressure Liquid Chromatography in Clinical Chemistry
Authors:
Carlson, R.E. and David Dolphin
Journal:
P.F. Dixon, C.H. Gray, C.K. Lim and M.S. Stoll, Eds., Academic Press, San Francisco, Ca.
Year:
1976.
Pages:
Abstract:
The porphyrias are a group of metabolic diseases characterized by the abnormal production and excretion of porphyrins. The clinical diagnosis of porphyria is based on the qualitative and quantitative determination of the pattern of excreted porphyrins which has been shown to be characteristic of the type of porphyria. A comparison of standard and urine samples on Corasil II and Porasil T demonstrates that Porasil T gives the better resolution in the 4 to 8 carboxyl region and is well suited to the analysis of urine samples in which the 4 to 8 carboxyl porphyrins is important. For the analysis of fecal samples in which the 2 to 4 carboxyl porphyrins dominate Corasil II is prefered but for routine clinical needs either column is equally suitable. A comparison of standard and urine samples on Corasil II and Porasil T demonstrates that Porasil T gives the better resolution in the 4 to 8 carboxyl region and is well suited to the analysis of urine samples in which the 4 to 8 carboxyl porphyrins is important. For the analysis of fecal samples in which the 2 to 4 carboxyl porphyrins dominate Corasil II is prefered but for routine clinical needs either column is equally suitable.

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